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KMID : 0606320050220010011
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Journal of Phamacetical Sciences Sookmyung Women s University
2005 Volume.22 No. 1 p.11 ~ p.15
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Effect of pH on Polyphenol Oxidase from Nicotiana tabacum L. leaves
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Kim An-Keun
Lee Soo-Yeun
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Abstract
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Abstract : We investigated the effect of pH on the polyphenol oxidase (PPO) activity in the cell wall, plastid, mitochondrial and soluble fractions of the leaves from Nicotiana tabacum L. PPO-catalized oxidation of tert-buthylcatechol were performed in the pH range 3-9. The optimal pH of PPO in the cell wall and mitochondrial fraction are 5.0 and 6.5 respectively, the optimal pH of PPO in both plastid and soluble fractions are 7.0.
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KEYWORD
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